Hunter Biden's lawyers sent letters singling out Trump allies and Fox News host Tucker Carlson.
Among the recipients are the Justice Department and Deleware attorney general, WaPo reported.
Attorneys are also seeking a retraction of "defamatory" statements from Fox News and Tucker Carlson.
Hunter Biden's lawyers are targeting Donald Trump's allies and Fox News in perhaps their most notable parry against the spate of attacks from conservatives who have fixated on the contents of Biden's laptop over the past few years.
Letters from Biden's lawyers show the beleaguered president's son is seeking criminal probes into people who may have had access to his computer data, including Trump's former personal lawyer Rudy Giuliani.
Two letters from Abbe Lowell, one of Biden's lawyers, were sent on Wednesday to the Justice Department and the Delaware attorney general, The Washington Post reported.
The letter to the Justice Department asked for an investigation into "individuals for whom there is considerable reason to believe violated various federal laws in accessing, copying, manipulating, and/or disseminating Mr. Biden's personal computer data," according to an NBC News report.
The Delaware attorney general was also tapped because Biden's lawyers believe the individuals may have violated state laws by accessing the computer, which was reportedly left at a repair shop in Wilmington.
According to The Post, key individuals singled out in the series of letters include John Paul Mac Isaac, the owner of the Wilmington repair shop; Giuliani, who admitted to sending a copy of the computer hard drive to The New York Post; Giuliani's lawyer Robert Costello; and Stephen Bannon, who had access to the laptop contents.
"This marks a new approach by Hunter Biden and his team," a person familiar with Biden's strategy told The Post. "He is not going to sit quietly by as questionable characters continue to violate his rights and media organizations peddling in lies try to defame him."
Also targeted by Biden's lawyers is Fox News and its host Tucker Carlson. Bryan M. Sullivan, another member of Biden's litigation team, accused the outlet and host of defamation and demanded the retraction of a story related to Biden's financial transactions, The Post reported.
"We demand that you immediately retract these statements by spending a significant of amount of air-time on such retraction," Sullivan wrote in the letters, according to The Post.
The Justice Department and Delaware attorney general's office did not immediately respond to a request for comment.